| Assay Method Information | |
| | ERRgamma FRET Assay |
| Description: | Recruitment of the RIP140 NR-box peptide to the ERRgamma ligand binding domain was assayed by FRET detection. Proteins were set up in a one to one ratio of Europium labeled streptavidin-ERRgamma: APC labeled streptavidin-RIP140 Complex. The proteins were incubated for 30 minutes then excess biotin was added to fill vacant streptavidin (SA) sites. Protein mixture was added to prepared plates. They were then counted on the Wallac Victor, and counts were then analyzed. |
| Affinity data for this assay | |
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