Assay Method Information

Assay Name:  Lactate Dehydrogenase Inhibition Assay
Description:  LDH was assayed spectrophotometrically for reduction of pyruvate using NADH by recording the changed in absorbance at 340 nm. The reaction was initiated by the addition of NADH, and the decrease in absorbance at 340 nm was monitored for 5 min. The compounds showing inhibition of more than 50% at 50 ug/mL concentration were retested and their IC50 values were determined.
Affinity data for this assay
 

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