| Assay Method Information | |
| | Tritiated Niacin Binding Assay and [35S]-GTP-gammaS Binding Assay |
| Description: | Membranes were incubated in binding buffer with [5, 6-3H]-niacin in the presence of test compound. After 4 hours at room temperature, reactions were filtered through Packard Unifilter GF/C plates using a Packard 96-well Harvester, and the bound radioactivity was measured using a Packard TopCount scintillation counter. Non-specific binding was determined in the presence of 250 uM unlabeled niacin. EC50/IC50 values were obtained from [35S]-GTP-gammaS binding assay using membranes prepared from Chinese Hamster Ovary (CHO)-K1 cells stably expressing GPR109A or vector control. |
| Affinity data for this assay | |
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