| Assay Method Information | |
| | Fluorometric Assay |
| Description: | The effectiveness of compounds against the activity of human recombinant caspase-1-8 was measured using fluorometric assays. Assays were carried out in a 96-well flat bottom, polystyrene plates. Caspase activity was monitored using a Microplate Spectrofluorometer Gemini XS with an excitation wavelength of 365 nm and an emission wavelength of 495 nm. Kinetic data were collected over a 15 min assay run at room temperature. IC50 values were calculated through a four parameter fit curve using the computer application SOFTmax PRO. Ki(apparent) values were calculated using the following equation: Ki(app) = IC50/(1 + [substrate]/Km). |
| Affinity data for this assay | |
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