Assay Method Information

Assay Name:  Solid-Phase ELISA Kinase Assay
Description:  The 96-well flat-bottomed plates were coated with recombinant GST-BAD. After the plates were blocked, the reaction buffer containing test compound and recombinant GST-PIM1 kinase were added to each well. Phosphorylated GST-BAD was detected by an ELISA reaction. The level of phosphorylated GST-BAD present was proportional to the absorbance at 450 nm. Curve fitting and enzyme analyses were done using GraphPad Prism version 4.00 (GraphPad Software, San Diego, CA).
Affinity data for this assay
 

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