Assay Method Information

Assay Name:  Inhibition of CARM-1 Enzymatic Activity
Description:  Histone H3 is used as the substrate for CARM-1 enzyme, and the methylation is monitored using tritiated S-Adenosyl-Methionine (SAM) as a methyl donor. The enzyme mixture containing test compound is left to pre-incubate for 10 minutes, followed by the addition of Histone H3/[3H] SAM mixture. After incubation, the mixture is spotted onto P30 Filtermat paper. The incorporated radioactivity is read using a Wallac Microbeta counter. The data is analyzed to generate IC50 values.
Affinity data for this assay
 

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