Assay Method Information

Assay Name:  Protease Inhibition Assay
Description:  IC50 values were obtained by assaying the enzyme against the synthetic substrate peptide KQGTVSFNFPQIT, which was tritiated at the proline residue, and then was coupled via its N-terminal lysine to an Affi-gel bead mixture. The assay was performed in 96-well microtiter filtration plates. The plates containing HIV protease and radiolabeled substrate beads were incubated with or without inhibitor. After filtration, radioactivity was measured using an LKB 1205 Microbeta liquid scintillation counter. IC50 determinations were performed in duplicate at each concentration with mean values used for data analysis.
Affinity data for this assay
 

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