| Assay Method Information | |
| | Protease Inhibition Assay |
| Description: | Sensitivity of HIV-1 protease activity to protease inhibitors was determined by a peptide substrate cleavage assay. Cleavage products and substrate were separated by reversed-phase HPLC on a Perkin-Elmer 3 X 3CR C8 column. A nonlinear curve fit using the Hill model was applied to the percent inhibition concentration data, and 50% effective concentrations (IC50) were calculated through the use of SAS (Statistical Software System, SAS Institute Inc., Cary, NC). |
| Affinity data for this assay | |
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