Assay Method Information

Assay Name:  Fluorescence Polarization Assay
Description:  Fluorescence polarization was measured on an Ultra plate reader (Tecan) at excitation and emission wavelengths of 485 and 530 nm, respectively. The equilibrium binding curves were drawn by plotting millipolarization units (mP) as a function of recombinant XIAP concentration. All experiments were performed in black, flat-bottomed, 96-well microplates. The Smac mimetics were evaluated for their ability to displace the fluorescent probe from recombinant protein. Data were analyzed using Prism 4.0 software (Graphpad Software).
Affinity data for this assay
 

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