Assay Method Information

Assay Name:  Enzyme Inhibition Assay (Ki) and Antiviral Activity Assay (EC50/IC50)
Description:  The Ki values were determined by substrate cleavage assay using fluorogenic substrate, 2-(aminobenzoyl)-Thr-Ile-Nle-Phe(p-NO2)-Gln-Arg-NH2. A standard curve relating changes in fluorescent intensity to changes in concentration of product was used to convert fluorescent changes into molar velocities. The amount of cleavage was determined by HPLC. IC50 values were determined by using the MTT assay. The IC50 values of saquinavir (SQV) and amprenavir (APV) tested as reference agents were 16 and 27 nM
Affinity data for this assay
 

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