| Assay Method Information | |
| | colorimetric ATPase assay |
| Description: | ATPase assay contained 10 mM Mg2+, 1uM 13/20 DNA, 500uM (r)NTP or (d)NTP, 500 nM gp45, and 500 nM gp44/62 in T4 buffer. ATPase activity was measured by monitoring the release of Pi using a malachite green assay (31) or via the hydrolysis of [gamma-32P]-ATP. Reaction inhibition studies used identical conditions except for the inclusion of variable concentrations of inhibitor (0-400 uM) and a fixed concentration of ATP (32.5 nM [gamma-32P]-ATP and 100 uM unlabeled ATP). |
| Affinity data for this assay | |
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