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Assay Method Information

Assay Name:  In vitro MKP-3 Phosphatase Dose Response Active/Probe Assessment Assay - Effects of Catalase
Description:  The MKP-3 dose response Active/Probe assessment-Catalase assay has been developed to evaluate the effects of adding 100 U/mL of Catalase on the MKP-3 inhibition of actives identified in the MKP-3 HTS run at (Burham Institute) SDCCG center AID 425 and the MKP-1 HTS run at the PMLSC AID 374, and subsequently confirmed in the MKP-3 & MKP-1 HTS dose response confirmation assays AID's 553 & 551. Protein tyrosine phosphatases have an active site cysteine that is very susceptible to inactivation by oxidation. In addition, a number of compounds such as quinone-like compounds are capable of generating reactive oxygen species via redox cycling in the presence of DTT. Adding Catalase to inactivate hydrogen peroxide (H2O2)does not affect the activity of MKP-3 in the assay but can reverse the inhibition of some inhibitors or significantly increase their IC50 values. The MKP-3 Phosphatase Dose Response Active/Probe Assessment Assay - Effects of Catalase has been Developed and Run at the Univers
Affinity data for this assay
 

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Last update November 1, 2007
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