| Assay Method Information | |
| | GAPDH Dose Response Colorimetric Assay |
| Description: | Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None This glyceraldehydes-3-phosphate dehydrogenase (GAPDH; EC 1.2.1.12) dose-response assay is developed and performed at the Sanford-Burnham Center for Chemical Genomics for characterization of the hits of biochemical assays. GAPDH is found in all mammalian tissues, and is considered a "housekeeping enzyme" unaffected by most physiological, hormonal, and metabolic changes. Therefore, compounds that inhibit GAPDH are expected to have pronounced cytotoxic effect and would be unfavorable as chemical probes. In addition, GAPDH has a labile cysteine in its active site that is crucial for the catalysis, resembling several other enzyme groups, such as cysteine-based proteases and phosphatases. As a result, the compounds capable of cysteine oxidation or mod |
| Affinity data for this assay | |
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