| Assay Method Information | |
| | Concentration Response Redox Cycling H2O2 Generation assay, 1 mM DTT, to characterize small molecule inhibitors identified in the Polo box domain (PBD) of Plk1 Primary HTS. |
| Description: | List of compounds to be tested: Compounds that met the active criterion of Z-score is </= -3 in the in vitro PLK1-PBD binding primary screen AID 693, will be tested in 10-point concentration response assays in the presence of 1.0 mM DTT. The PLK-1-PBD hit characterization compound concentration dependent redox cycling H2O2 generation assay in the presence of 1.0 mM DTT, has been developed to evaluate actives that were identified in the in vitro PLK1-PBD binding primary screen AID 693, conducted by the University of Pittsburgh Molecular Screening Center (PMLSC) part of the Molecular Library Screening Center Network (MLSCN). MH078944 "High throughput fluorescence polarization-based assay to screen for small molecule inhibitors of the Polo box domain (PBD) of Plk1" proposal submitted by Dr. Michael Yaffe of MIT. The compound dose dependent redox cycling H2O2 generation assay was developed to determine whether any of the PLK-1-PBD inhibitor compounds might be indirect binding inhibit |
| Affinity data for this assay | |
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