Assay Method Information | |
| Fluorescence Cell-Based Secondary Assay to Identify Inhibitors of Calcineurin |
Description: | Keywords: Candida albicans, drug resistance, Fluconazole, calcineurin, stress response Assay Overview: Method for determining if compound acts as Calcineurin inhibitor. S cerevisiae expressing beta-galactosidase driven by 4 tandem copies of the calcineurin-dependent response element (CDRE) is exposed to compounds followed by challenge with CaCl2 stressor. Potential inhibition of calcineurin function will be evaluated by loss of signal. Expected Outcome: Reduction of signal indicates calcineurin inhibition by compound. |
Affinity data for this assay | |
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