| Assay Method Information | |
| | Dose response confirmation uHTS hits for MazEF TA System activators via a fluorescence-based single-stranded RNase assay |
| Description: | Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Probe Production Centers Network (MLPCN) Grant Number: 2R01 GM068385-06 Assay Provider: Dr. Paul Hergenrother, University of Illinois, Urbana, IL Bacterial resistance to antibiotics is a worldwide health crisis. Resistance typically occurs as a result of chromosomal mutation or acquisition of a mobile genetic element, such as a plasmid, that harbors resistance-mediating genes. One of the most common plasmid maintenance systems is the toxin-antitoxin (TA) postsegregational killing mechanism. In this mechanism, if a plasmid-free daughter cell arises, the labile antitoxin is degraded and the toxin induces cell death. TA genes are ubiquitous in clinical isolates of certain drug-resistant bacteria, and it has been postulated that compounds that disrupt the TA interaction could free the toxin to kill the bac |
| Affinity data for this assay | |
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