| Assay Method Information | |
| | Dose Response of Small Molecules that Regulate V-ATPase Proton Transport in Yeast using pHLuorin, Powder Set1 |
| Description: | University of New Mexico Assay Overview: Assay Support: 1 R03 DA031666-01A1 Project Title: Flow Cytometry HTS of Small Molecules that Regulate V-ATPase Proton Transport in Yeast Assay Provider: Karlett Parra Ph.D Lead Biologist: Mark Carter MS Chemistry Center/ PI: Specialized Chemistry Center: Assay Implementation: Mark Carter, Chun-Yuan Chan, Stephanie Chavez, Dominique Perez, Matthew Garcia, Terry Foutz, Anna Waller Ph.D, Annette Evangelisti Ph.D, Gergely Zahoransky-Kohalmi Assay Background and Significance: Distributed among the endomembrane system of all eukaryotic cells, V-ATPase proton pumps are responsible for acidification of intracellular compartments. V-ATPases maintain the low pH necessary for endocytic and exocytic vesicular transport, zymogen activation, and protein sorting and degradation. Enveloped viruses, such as influenza virus, as well as toxins, such as diphtheria toxin, enter cells via acidic endosomal compartments, in which low pH is maintained by V-ATPases. |
| Affinity data for this assay | |
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