| Assay Method Information | |
| | Antimicrobial Susceptibility Testing Assay |
| Description: | The in vitro antimicrobial activity of the compounds was alternatively determined according to the following procedure:The assay used a 10-points Iso-Sensitest broth medium to measure quantitatively the in vitro activity of the compounds against A. baumannii ATCC 17978.Stock compounds in DMSO were serially twofold diluted (e.g. range from 50 to 0.097 μM final concentration) in 384 wells microtiter plates and inoculated with 49 μl the bacterial suspension in Iso-Sensitest medium to have a final cell concentration of 5×10(5) CFU/ml in a final volume/well of 50 ul/well. Microtiter plates were incubated at 35±2° C.Bacterial cell growth was determined with the measurement of optical density at λ=600 nm each 20 minutes over a time course of 16 h.Growth inhibition was calculated during the logarithmic growth of the bacterial cells with determination of the concentration inhibiting 50% (IC50) and 90% (IC90) of the growth.Tables 3, 4 and 5 provide the 50% growth inhibitory concentrations (IC50) in micromoles per liter of the compounds of present invention obtained against the A. baumannii strain ATCC17978. |
| Affinity data for this assay | |
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