Assay Method Information

Assay Name:  AR Activity
Description:  The reaction mixture contained 1 mL of 1 M potassium phosphate buffer (pH 6.2), 0.4 mM lithium sulfate and 5 µM 2-mercaptoethanol, 10 µM DL-glyceraldehyde, 0.1 µM NADPH, and crude enzyme. The reaction mixture was incubated at 37 °C for 10 min. The reaction was initiated by the addition of NADPH and the decrease in absorbance was measured at 340 nm.
Affinity data for this assay
 

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