Assay Method Information

Assay Name:  Fluorometric Activity Assay
Description:  Enzymatic activity was determined with fluorogenic substrate in the presence and absence of inhibitor. The fluorescence increase due to hydrolysis of the substrate was monitored with an excitation filter of wavelength 340 nm and an emission filter of wavelength 420. Values for the inhibition constants (Ki) were determined by conventional steady-state kinetic techniques.
Affinity data for this assay
 

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