Assay Method Information

Assay Name:  In Vitro PTP1B Inhibition Assay
Description:  PTP1B tyrosine phosphatise drug discovery kit (BML-AK 822, Enzo life sciences, USA) was utilized for in vitro testing of synthesized compounds against PTP1B. 10 mM stock solution (in assay buffer containing 1% DMSO) of the test compounds were prepared. Taking suitable aliquots from this stock solution, five different concentrations were made by dilutions. The test compounds along with suramin(standard inhibitor) were incubated with human recombinant PTP1B enzyme and then free phosphate was measured using biomol red as phosphate detection reagent. A 96 well microtiter plate was used for carrying out the assay using 10 mM DMSO as control. The reaction was carried out in 96 well, flatbottomed microtiter plates at 10 mM concentration using DMSO as control.
Affinity data for this assay
 

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