| Assay Method Information | |
| | PTP1B Inhibitory Assay |
| Description: | PTP1B activity was measured by adding 2mM p-NPP and PTP1B in a 50 mM citrate buffer (pH 6.0, 0.1 M NaCl, 1 mMEDTA, and 1 mM dithiothreitol), with or without tested compounds. The plate was pre-incubated at 37 °C for 10 min, and then 50 μL of p-NPP in buffer was added. After incubating at 37 °C for 30 min, the reaction was then terminated with 1 N NaOH. The amount of produced p-nitrophenyl after enzymatic dephosphorylation was obtained by measuring the absorbance at 405 nm using a VersaMax microplate reader (Molecular Devices, Sunnyvale, CA, USA). The non-enzymatic reactions of 2 mM p-NPP were determined by measuring the increase in absorbance at 405 nm without PTB1B enzyme. |
| Affinity data for this assay | |
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