Assay Method Information

Assay Name:  MT Kinetic Assay
Description:  The assay was performed as previously described with slight modifications [Chen et al., J. Agric. Food Chem., 50:4108-12]. The reaction medium was 1 mL in 50 mM phosphate buffer (pH 6.8). The final concentration of mushroom tyrosinase was 26.07 μg/mL for cresolase activity and 6.52 μg/mL for catecholase activity. Freshly prepared enzyme, substrate, and dithiocarbamates I and II were used in this work. The reaction was carried out under constant temperatures of 27 and 37°C. Substrate addition followed incubation of the enzyme with different concentrations of synthetic inhibitors.
Affinity data for this assay
 

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