Assay Method Information

Assay Name:  CA Enzyme Assay
Description:  CA activity was measured by the Maren method that is based on determination of the time required for the pH to decrease from 10.0 to 7.4 due to CO2 hydration. Phenolred was added to the assay medium as the pH indicator, and the buffer was 0.5 M Na2CO3/0.1 M NaHCO3 (pH 10.0). One unit of CA activity is defined as the amount ofthe enzyme that reduces by 50% the time of CO2 hydration measured in the absence of enzyme. In the inhibition studies, the CO2 concentration was 70 mM and at last five different inhibitor concentrations were used.
Affinity data for this assay
 

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