Assay Method Information

Assay Name:  HSP70 Biacore Direct Binding Assay
Description:  All experiments were performed on a Biacore T100 at 25 deg C with a flow rate of 30 uL per minute. Double His-tagged HSP70 was immobilised on the surface of a NTA sensor chip; approximately 2000 RU of protein were immobilised. Compounds were injected for 90 sec, and the KDs were determined from equilibrium binding at 80 sec.
Affinity data for this assay
 

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