| Assay Method Information | |
| | Fluorescent Coupled Assay |
| Description: | The binding affinities to FtsZ were determined by a fluorescent coupled assay for phosphate relase. In this GTPase studies, phosphate liberated during GTP hydrolysis is a substrate of purine nucleoside phosphorylase, which converts 7-methylguanosine to 7-methylguanin and ribose-1-phosphate, resulting in a decrease of fluorescence. |
| Affinity data for this assay | |
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