| Assay Method Information | |
| | Isothermal Titration Calorimetry (ITC) |
| Description: | The purified EphA proteins (EphA4-LBD, EphA3-LBD, and EphA2-LBD) were dissolved in 50 mM potassium phosphate buffer (pH 6.5) containing 100 mM NaCl. Compound binding was detected at 27 °C by comparing either the 2D-[15N,1H]-HSQC or 2D-[13C,1H]-HSQC spectra of 50 mMEphA4-LBD in absence and presence of compounds at 50 mM. ITC were measured with Model ITC200 from Microcal/GE Life Sciences. |
| Affinity data for this assay | |
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