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Assay Method Information

Assay Name:  ChEMBL_1619388
Description:  Inhibition of recombinant Yersinia pestis C-terminal His6-tagged HMWP2 cysteine adenylation domain (1 to 1491 residues) expressed in Escherichia coli BL21(DE3) using 7-Methyl-6-thioguanosine as substrate preincubated for 10 mins followed by L-cysteine addition measured for 50 mins in presence of purine nucleoside phosphorylase/inorganic pyrophosphatase by spectrophotometric method
Affinity data for this assay
 

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Last update November 1, 2007
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