| Assay Method Information | |
| | In Vitro AMSH Activity Assay |
| Description: | AMSH activity was measured using 10 nM purified AMSH (E-548B, Boston Biochem) and 80 nM di-ubiquitin substrate DiUbK63TAMRA (UF-310, Boston Biochem). TAMRA fluorescence intensity was monitored using a PHERAstar plate reader with excitation at 540 nm and emission at 590 nm. Reactions were performed in black low-volume 384-well plates and analyzed as described above. |
| Affinity data for this assay | |
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