| Assay Method Information | |
| | Z′-LYTE assay |
| Description: | BMX, TXK, EGFR, ERBB2, ERBB4, JAK3, BLK kinase activity was measured using the Z′-LYTE assay at Thermo Fisher. A 10-point dose response (final concentration in assay ranged from 10 μM to 0.5 nM in 3-fold dilution per dilution step) was generated with 1 h incubation of the test compound with the kinase prior to initiation of the kinase reaction by the addition of ATP. ATP concentration in the assay was Km ATP for the different kinases. IC50 values are determined by curve fitting of the experimental results at Thermo Fisher. |
| Affinity data for this assay | |
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