Abstract
Two CMY-2 derivatives, CMY-31 (Gln(215)-->Arg) from Salmonella enterica serotype Newport and CMY-36 (Ala(77)-->Cys and Gln(193)-->Glu) from Klebsiella pneumoniae, were characterized. Both cephalosporinases functionally resembled CMY-2. bla(CMY) alleles occurred as parts of a putative transposon comprising ISEcp1B and a Citrobacter freundii-derived sequence carried by ColE1-like plasmids similar to CMY-5-encoding pTKH11 from Klebsiella oxytoca.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Alleles
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Amino Acid Sequence
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Amino Acid Substitution
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Arginine / metabolism
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Base Sequence
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Cephalosporinase / genetics*
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Citrobacter freundii / enzymology
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Cysteine / metabolism
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DNA Transposable Elements
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DNA, Bacterial / genetics
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Escherichia coli / genetics
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Genes, Bacterial
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Glutamine / metabolism
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Humans
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Hydrolysis
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Inhibitory Concentration 50
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Isoelectric Point
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Kinetics
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Klebsiella pneumoniae / genetics
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Klebsiella pneumoniae / isolation & purification
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Microbial Sensitivity Tests
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Models, Genetic
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Models, Molecular
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Molecular Sequence Data
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Plasmids*
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Salmonella enterica / classification
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Salmonella enterica / genetics
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Salmonella enterica / isolation & purification
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Sequence Analysis, DNA
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Species Specificity
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Substrate Specificity
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Transformation, Genetic
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beta-Lactamase Inhibitors
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beta-Lactamases / genetics*
Substances
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DNA Transposable Elements
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DNA, Bacterial
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beta-Lactamase Inhibitors
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Glutamine
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Arginine
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Cephalosporinase
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beta-Lactamases
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Cysteine