Affinity DataIC50: 0.300nMAssay Description:Briefly, 20 nL of compounds were transferred to the 384-well plate by Echo 550 liquid handler (Labcyte, USA), then 5 μL of BRD4(BD1) (Reaction B...More data for this Ligand-Target Pair
Affinity DataIC50: 0.340nMAssay Description:Briefly, 20 nL of compounds were transferred to the 384-well plate by Echo 550 liquid handler (Labcyte, USA), then 5 μL of BRD4(BD1) (Reaction B...More data for this Ligand-Target Pair
Affinity DataIC50: 0.350nMAssay Description:Briefly, 20 nL of compounds were transferred to the 384-well plate by Echo 550 liquid handler (Labcyte, USA), then 5 μL of BRD4(BD1) (Reaction B...More data for this Ligand-Target Pair
Affinity DataIC50: 0.400nMAssay Description:Briefly, 20 nL of compounds were transferred to the 384-well plate by Echo 550 liquid handler (Labcyte, USA), then 5 μL of BRD4(BD1) (Reaction B...More data for this Ligand-Target Pair
Affinity DataIC50: 0.480nMAssay Description:Briefly, 20 nL of compounds were transferred to the 384-well plate by Echo 550 liquid handler (Labcyte, USA), then 5 μL of BRD4(BD1) (Reaction B...More data for this Ligand-Target Pair
Affinity DataIC50: 0.490nMAssay Description:Briefly, 20 nL of compounds were transferred to the 384-well plate by Echo 550 liquid handler (Labcyte, USA), then 5 μL of BRD4(BD1) (Reaction B...More data for this Ligand-Target Pair
Affinity DataIC50: 0.540nMAssay Description:Briefly, 20 nL of compounds were transferred to the 384-well plate by Echo 550 liquid handler (Labcyte, USA), then 5 μL of BRD4(BD1) (Reaction B...More data for this Ligand-Target Pair
Affinity DataIC50: 0.550nMAssay Description:Briefly, 20 nL of compounds were transferred to the 384-well plate by Echo 550 liquid handler (Labcyte, USA), then 5 μL of BRD4(BD1) (Reaction B...More data for this Ligand-Target Pair
Affinity DataIC50: 0.570nMAssay Description:Briefly, 20 nL of compounds were transferred to the 384-well plate by Echo 550 liquid handler (Labcyte, USA), then 5 μL of BRD4(BD1) (Reaction B...More data for this Ligand-Target Pair
Affinity DataIC50: 0.590nMAssay Description:Briefly, 20 nL of compounds were transferred to the 384-well plate by Echo 550 liquid handler (Labcyte, USA), then 5 μL of BRD4(BD1) (Reaction B...More data for this Ligand-Target Pair
Affinity DataIC50: 0.610nMAssay Description:Briefly, 20 nL of compounds were transferred to the 384-well plate by Echo 550 liquid handler (Labcyte, USA), then 5 μL of BRD4(BD1) (Reaction B...More data for this Ligand-Target Pair
Affinity DataIC50: 0.640nMAssay Description:In the present application, the IC50 value of the compound of formula (I) in inhibiting BRD4 (BD1) enzyme binding reaction were determined by homogen...More data for this Ligand-Target Pair
Affinity DataIC50: 0.660nMAssay Description:Briefly, 20 nL of compounds were transferred to the 384-well plate by Echo 550 liquid handler (Labcyte, USA), then 5 μL of BRD4(BD1) (Reaction B...More data for this Ligand-Target Pair
Affinity DataIC50: 0.680nMAssay Description:Briefly, 20 nL of compounds were transferred to the 384-well plate by Echo 550 liquid handler (Labcyte, USA), then 5 μL of BRD4(BD1) (Reaction B...More data for this Ligand-Target Pair
Affinity DataIC50: 0.790nMAssay Description:Briefly, 20 nL of compounds were transferred to the 384-well plate by Echo 550 liquid handler (Labcyte, USA), then 5 μL of BRD4(BD1) (Reaction B...More data for this Ligand-Target Pair
Affinity DataIC50: 0.880nMAssay Description:Briefly, 20 nL of compounds were transferred to the 384-well plate by Echo 550 liquid handler (Labcyte, USA), then 5 μL of BRD4(BD1) (Reaction B...More data for this Ligand-Target Pair
Affinity DataIC50: 0.970nMAssay Description:Briefly, 20 nL of compounds were transferred to the 384-well plate by Echo 550 liquid handler (Labcyte, USA), then 5 μL of BRD4(BD1) (Reaction B...More data for this Ligand-Target Pair
Affinity DataIC50: 0.990nMAssay Description:Briefly, 20 nL of compounds were transferred to the 384-well plate by Echo 550 liquid handler (Labcyte, USA), then 5 μL of BRD4(BD1) (Reaction B...More data for this Ligand-Target Pair
Affinity DataIC50: 1.10nMAssay Description:Briefly, 20 nL of compounds were transferred to the 384-well plate by Echo 550 liquid handler (Labcyte, USA), then 5 μL of BRD4(BD1) (Reaction B...More data for this Ligand-Target Pair
Affinity DataIC50: 1.30nMAssay Description:Briefly, 20 nL of compounds were transferred to the 384-well plate by Echo 550 liquid handler (Labcyte, USA), then 5 μL of BRD4(BD1) (Reaction B...More data for this Ligand-Target Pair
Affinity DataIC50: 1.60nMAssay Description:Briefly, 20 nL of compounds were transferred to the 384-well plate by Echo 550 liquid handler (Labcyte, USA), then 5 μL of BRD4(BD1) (Reaction B...More data for this Ligand-Target Pair
Affinity DataIC50: 1.90nMAssay Description:Briefly, 20 nL of compounds were transferred to the 384-well plate by Echo 550 liquid handler (Labcyte, USA), then 5 μL of BRD4(BD1) (Reaction B...More data for this Ligand-Target Pair
Affinity DataIC50: 2nMpH: 7.5 T: 2°CAssay Description:The labeled ligand specifically binds BRD4-1 and BRD4-2 and can be displaced by a small molecule inhibitor that shares a similar or overlapping bindi...More data for this Ligand-Target Pair
Affinity DataIC50: 2.19nMAssay Description:All reagents were diluted in 50 mM HEPES, 100 mM NaCl, 0.1% BSA, pH 7.4 supplemented with 0.05% CHAPS and allowed to equilibrate to room temperature ...More data for this Ligand-Target Pair
Affinity DataIC50: 2.20nMAssay Description:Briefly, 20 nL of compounds were transferred to the 384-well plate by Echo 550 liquid handler (Labcyte, USA), then 5 μL of BRD4(BD1) (Reaction B...More data for this Ligand-Target Pair
Affinity DataIC50: 2.49nMAssay Description:All reagents were diluted in 50 mM HEPES, 100 mM NaCl, 0.1% BSA, pH 7.4 supplemented with 0.05% CHAPS and allowed to equilibrate to room temperature ...More data for this Ligand-Target Pair
Affinity DataIC50: 3nMAssay Description:Briefly, 20 nL of compounds were transferred to the 384-well plate by Echo 550 liquid handler (Labcyte, USA), then 5 μL of BRD4(BD1) (Reaction B...More data for this Ligand-Target Pair
Affinity DataIC50: 3.48nMAssay Description:All reagents were diluted in 50 mM HEPES, 100 mM NaCl, 0.1% BSA, pH 7.4 supplemented with 0.05% CHAPS and allowed to equilibrate to room temperature ...More data for this Ligand-Target Pair
Affinity DataIC50: 3.80nMpH: 7.5 T: 2°CAssay Description:The labeled ligand specifically binds BRD4-1 and BRD4-2 and can be displaced by a small molecule inhibitor that shares a similar or overlapping bindi...More data for this Ligand-Target Pair
Affinity DataIC50: 4nMpH: 7.5 T: 2°CAssay Description:The labeled ligand specifically binds BRD4-1 and BRD4-2 and can be displaced by a small molecule inhibitor that shares a similar or overlapping bindi...More data for this Ligand-Target Pair
Affinity DataIC50: 4.60nMAssay Description:Briefly, 20 nL of compounds were transferred to the 384-well plate by Echo 550 liquid handler (Labcyte, USA), then 5 μL of BRD4(BD1) (Reaction B...More data for this Ligand-Target Pair
Affinity DataIC50: 4.67nMAssay Description:Briefly, 20 nL of compounds were transferred to the 384-well plate by Echo 550 liquid handler (Labcyte, USA), then 5 μL of BRD4(BD1) (Reaction B...More data for this Ligand-Target Pair
Affinity DataIC50: 4.70nMpH: 7.5 T: 2°CAssay Description:The labeled ligand specifically binds BRD4-1 and BRD4-2 and can be displaced by a small molecule inhibitor that shares a similar or overlapping bindi...More data for this Ligand-Target Pair
Affinity DataIC50: 5.40nMpH: 7.5 T: 2°CAssay Description:The labeled ligand specifically binds BRD4-1 and BRD4-2 and can be displaced by a small molecule inhibitor that shares a similar or overlapping bindi...More data for this Ligand-Target Pair
Affinity DataIC50: 5.90nMpH: 7.5 T: 2°CAssay Description:The labeled ligand specifically binds BRD4-1 and BRD4-2 and can be displaced by a small molecule inhibitor that shares a similar or overlapping bindi...More data for this Ligand-Target Pair
Affinity DataIC50: 7.20nMpH: 7.5 T: 2°CAssay Description:The labeled ligand specifically binds BRD4-1 and BRD4-2 and can be displaced by a small molecule inhibitor that shares a similar or overlapping bindi...More data for this Ligand-Target Pair
Affinity DataIC50: 10.4nMpH: 7.5 T: 2°CAssay Description:The labeled ligand specifically binds BRD4-1 and BRD4-2 and can be displaced by a small molecule inhibitor that shares a similar or overlapping bindi...More data for this Ligand-Target Pair
Affinity DataIC50: 10.7nMpH: 7.5 T: 2°CAssay Description:The labeled ligand specifically binds BRD4-1 and BRD4-2 and can be displaced by a small molecule inhibitor that shares a similar or overlapping bindi...More data for this Ligand-Target Pair
Affinity DataIC50: 10.8nMpH: 7.5 T: 2°CAssay Description:The labeled ligand specifically binds BRD4-1 and BRD4-2 and can be displaced by a small molecule inhibitor that shares a similar or overlapping bindi...More data for this Ligand-Target Pair
Affinity DataIC50: 12nMAssay Description:Briefly, 20 nL of compounds were transferred to the 384-well plate by Echo 550 liquid handler (Labcyte, USA), then 5 μL of BRD4(BD1) (Reaction B...More data for this Ligand-Target Pair
Affinity DataIC50: 12.3nMpH: 7.5 T: 2°CAssay Description:Binding of the bromodomain BRD4-I to an acetylated histone H4 peptide was measured using a bead-based Amplified Luminescent Proximity Homogeneous Ass...More data for this Ligand-Target Pair
Affinity DataIC50: 14.3nMpH: 7.5 T: 2°CAssay Description:Binding of the bromodomain BRD4-I to an acetylated histone H4 peptide was measured using a bead-based Amplified Luminescent Proximity Homogeneous Ass...More data for this Ligand-Target Pair
Affinity DataIC50: 14.4nMpH: 7.5 T: 2°CAssay Description:Binding of the bromodomain BRD4-I to an acetylated histone H4 peptide was measured using a bead-based Amplified Luminescent Proximity Homogeneous Ass...More data for this Ligand-Target Pair
Affinity DataIC50: 15.9nMpH: 7.5 T: 2°CAssay Description:Binding of the bromodomain BRD4-I to an acetylated histone H4 peptide was measured using a bead-based Amplified Luminescent Proximity Homogeneous Ass...More data for this Ligand-Target Pair
Affinity DataIC50: 17nMAssay Description:Briefly, 20 nL of compounds were transferred to the 384-well plate by Echo 550 liquid handler (Labcyte, USA), then 5 μL of BRD4(BD1) (Reaction B...More data for this Ligand-Target Pair
Affinity DataIC50: 18nMAssay Description:All reagents were diluted in 50 mM HEPES, 100 mM NaCl, 0.1% BSA, pH 7.4 supplemented with 0.05% CHAPS and allowed to equilibrate to room temperature ...More data for this Ligand-Target Pair
Affinity DataIC50: 18.1nMpH: 7.5 T: 2°CAssay Description:Binding of the bromodomain BRD4-I to an acetylated histone H4 peptide was measured using a bead-based Amplified Luminescent Proximity Homogeneous Ass...More data for this Ligand-Target Pair
Affinity DataIC50: 18.9nMpH: 7.5 T: 2°CAssay Description:Binding of the bromodomain BRD4-I to an acetylated histone H4 peptide was measured using a bead-based Amplified Luminescent Proximity Homogeneous Ass...More data for this Ligand-Target Pair
Affinity DataIC50: 19nMAssay Description:1. Add 20 μl of the diluted compound to each well of the 384-well opti plate2. Tap the plate gently3. Add 10 μl of the enzyme to each well ...More data for this Ligand-Target Pair
Affinity DataIC50: 22nMpH: 7.5 T: 2°CAssay Description:Binding of the bromodomain BRD4-I to an acetylated histone H4 peptide was measured using a bead-based Amplified Luminescent Proximity Homogeneous Ass...More data for this Ligand-Target Pair
