Affinity DataIC50: 0.574nMpH: 7.5 T: 2°CAssay Description:Assays were performed with minor modifications from the manufacturer's protocol (PerkinElmer, USA). All reagents were diluted in 50 mM HEPES, 150...More data for this Ligand-Target Pair
Affinity DataIC50: 0.878nMpH: 7.5 T: 2°CAssay Description:Assays were performed with minor modifications from the manufacturer's protocol (PerkinElmer, USA). All reagents were diluted in 50 mM HEPES, 150...More data for this Ligand-Target Pair
Affinity DataIC50: 1nMAssay Description:The AlphaScreen™ assay was performed following previous publication with minor modifications from the manufacturer's protocol (PerkinElmer, USA)....More data for this Ligand-Target Pair
Affinity DataIC50: 1nMAssay Description:The AlphaScreen™ assay was performed following previous publication with minor modifications from the manufacturer's protocol (PerkinElmer, USA)....More data for this Ligand-Target Pair
Affinity DataIC50: 1nMAssay Description:The AlphaScreen™ assay was performed following previous publication with minor modifications from the manufacturer's protocol (PerkinElmer, USA)....More data for this Ligand-Target Pair
Affinity DataIC50: 1.09nMpH: 7.5 T: 2°CAssay Description:Assays were performed with minor modifications from the manufacturer's protocol (PerkinElmer, USA). All reagents were diluted in 50 mM HEPES, 150...More data for this Ligand-Target Pair
Affinity DataIC50: 1.59nMpH: 7.5 T: 2°CAssay Description:Assays were performed with minor modifications from the manufacturer's protocol (PerkinElmer, USA). All reagents were diluted in 50 mM HEPES, 150...More data for this Ligand-Target Pair
Affinity DataIC50: 1.60nMAssay Description:Inhibition of recombinant human BRDT BD2 (250 to 382 residues) expressed in bacterial expression system by bromoscan assayMore data for this Ligand-Target Pair
Affinity DataIC50: 1.84nMpH: 7.5 T: 2°CAssay Description:Assays were performed with minor modifications from the manufacturer's protocol (PerkinElmer, USA). All reagents were diluted in 50 mM HEPES, 150...More data for this Ligand-Target Pair
Affinity DataIC50: 2.10nMAssay Description:The AlphaScreen™ assay was performed following previous publication with minor modifications from the manufacturer's protocol (PerkinElmer, USA)....More data for this Ligand-Target Pair
Affinity DataIC50: 2.36nMpH: 7.5 T: 2°CAssay Description:Assays were performed with minor modifications from the manufacturer's protocol (PerkinElmer, USA). All reagents were diluted in 50 mM HEPES, 150...More data for this Ligand-Target Pair
Affinity DataIC50: 3nMAssay Description:Inhibition of BRDT BD1 (unknown origin) using biotinylated JQ1 analog as substrate measured after 20 mins by AlphaLISA assayMore data for this Ligand-Target Pair
Affinity DataIC50: 3nMAssay Description:Inhibition of BRDT BD2 (unknown origin) using biotinylated JQ1 analog as substrate measured after 20 mins by AlphaLISA assayMore data for this Ligand-Target Pair
Affinity DataIC50: 4.70nMAssay Description:The AlphaScreen™ assay was performed following previous publication with minor modifications from the manufacturer's protocol (PerkinElmer, USA)....More data for this Ligand-Target Pair
Affinity DataIC50: 5.10nMAssay Description:The AlphaScreen™ assay was performed following previous publication with minor modifications from the manufacturer's protocol (PerkinElmer, USA)....More data for this Ligand-Target Pair
Affinity DataIC50: 5.40nMAssay Description:The AlphaScreen™ assay was performed following previous publication with minor modifications from the manufacturer's protocol (PerkinElmer, USA)....More data for this Ligand-Target Pair
Affinity DataIC50: 5.5nMAssay Description:Binding affinity to human partial length BRDT BD2 (K250 to E382 residues) expressed in bacterial expression system using H4K5acIC8acK12acK16ac-biotin...More data for this Ligand-Target Pair
Affinity DataIC50: 5.78nMpH: 7.5 T: 2°CAssay Description:Assays were performed with minor modifications from the manufacturer's protocol (PerkinElmer, USA). All reagents were diluted in 50 mM HEPES, 150...More data for this Ligand-Target Pair
Affinity DataIC50: 6.10nMpH: 7.5 T: 2°CAssay Description:Assays were performed with minor modifications from the manufacturer's protocol (PerkinElmer, USA). All reagents were diluted in 50 mM HEPES, 150...More data for this Ligand-Target Pair
Affinity DataIC50: 6.20nMpH: 7.5 T: 2°CAssay Description:Assays were performed with minor modifications from the manufacturer's protocol (PerkinElmer, USA). All reagents were diluted in 50 mM HEPES, 150...More data for this Ligand-Target Pair
TargetBromodomain testis-specific protein [44-116](Homo sapiens (Human))
Baylor College of Medicine
US Patent
Baylor College of Medicine
US Patent
Affinity DataIC50: 7.60nMAssay Description:The AlphaScreen™ assay was performed following previous publication with minor modifications from the manufacturer's protocol (PerkinElmer, USA)....More data for this Ligand-Target Pair
Affinity DataIC50: 7.90nMAssay Description:Inhibition of 6His-FLAG-Tev-BRDT (1 to 397 residues) BD2 domain Y309A or Y66A mutant (unknown origin) incubated for 30 mins by TR-FRET assayMore data for this Ligand-Target Pair
TargetBromodomain testis-specific protein [44-116](Homo sapiens (Human))
Baylor College of Medicine
US Patent
Baylor College of Medicine
US Patent
Affinity DataIC50: 10nMpH: 7.5 T: 2°CAssay Description:Assays were performed with minor modifications from the manufacturer's protocol (PerkinElmer, USA). All reagents were diluted in 50 mM HEPES, 150...More data for this Ligand-Target Pair
Affinity DataIC50: 11nMAssay Description:Inhibition of recombinant BRDT-BD1 (unknown origin) by TR-FRET assayMore data for this Ligand-Target Pair
Affinity DataIC50: 11nMAssay Description:Inhibition of recombinant BRDT-BD2 (unknown origin) by TR-FRET assayMore data for this Ligand-Target Pair
Affinity DataIC50: 11nMAssay Description:Inhibition of BRDT BD2 (unknown origin) incubated for 30 mins by fluorescence based microtiter plate reader assayMore data for this Ligand-Target Pair
Affinity DataIC50: 11nMAssay Description:Inhibition of BRDT BD1 (unknown origin) incubated for 30 mins by fluorescence based microtiter plate reader assayMore data for this Ligand-Target Pair
TargetBromodomain testis-specific protein [44-116](Homo sapiens (Human))
Baylor College of Medicine
US Patent
Baylor College of Medicine
US Patent
Affinity DataIC50: 12.1nMpH: 7.5 T: 2°CAssay Description:Assays were performed with minor modifications from the manufacturer's protocol (PerkinElmer, USA). All reagents were diluted in 50 mM HEPES, 150...More data for this Ligand-Target Pair
Affinity DataIC50: 13nMAssay Description:The compounds were screened in vitro, and each compound was serially diluted to 10 concentrations. Four proteins BRD4 (D1+D2), BRDT (D1), BRD2 (D1+D2...More data for this Ligand-Target Pair
Affinity DataIC50: 13nMAssay Description:The compounds were screened in vitro, and each compound was serially diluted to 10 concentrations. Four proteins BRD4 (D1+D2), BRDT (D1), BRD2 (D1+D2...More data for this Ligand-Target Pair
TargetBromodomain testis-specific protein [44-116](Homo sapiens (Human))
Baylor College of Medicine
US Patent
Baylor College of Medicine
US Patent
Affinity DataIC50: 13.2nMpH: 7.5 T: 2°CAssay Description:Assays were performed with minor modifications from the manufacturer's protocol (PerkinElmer, USA). All reagents were diluted in 50 mM HEPES, 150...More data for this Ligand-Target Pair
Affinity DataIC50: 13.6nMpH: 7.5 T: 2°CAssay Description:Assays were performed with minor modifications from the manufacturer's protocol (PerkinElmer, USA). All reagents were diluted in 50 mM HEPES, 150...More data for this Ligand-Target Pair
TargetBromodomain testis-specific protein [44-116](Homo sapiens (Human))
Baylor College of Medicine
US Patent
Baylor College of Medicine
US Patent
Affinity DataIC50: 14nMAssay Description:The AlphaScreen™ assay was performed following previous publication with minor modifications from the manufacturer's protocol (PerkinElmer, USA)....More data for this Ligand-Target Pair
Affinity DataIC50: 14nMpH: 7.5Assay Description:The assay was carried out in binding mixtures of the bromodomain protein, 0-10 μM compounds and tetra-acetylated histone peptide (SGRGAC.KGGAC.K...More data for this Ligand-Target Pair
TargetBromodomain testis-specific protein [44-116](Homo sapiens (Human))
Baylor College of Medicine
US Patent
Baylor College of Medicine
US Patent
Affinity DataIC50: 15nMAssay Description:The compounds were screened in vitro, and each compound was serially diluted to 10 concentrations. Four proteins BRD4 (D1+D2), BRDT (D1), BRD2 (D1+D2...More data for this Ligand-Target Pair
Affinity DataIC50: 15nMAssay Description:The compounds were screened in vitro, and each compound was serially diluted to 10 concentrations. Four proteins BRD4 (D1+D2), BRDT (D1), BRD2 (D1+D2...More data for this Ligand-Target Pair
Affinity DataIC50: 16nMAssay Description:The compounds were screened in vitro, and each compound was serially diluted to 10 concentrations. Four proteins BRD4 (D1+D2), BRDT (D1), BRD2 (D1+D2...More data for this Ligand-Target Pair
Affinity DataIC50: 16nMAssay Description:The compounds were screened in vitro, and each compound was serially diluted to 10 concentrations. Four proteins BRD4 (D1+D2), BRDT (D1), BRD2 (D1+D2...More data for this Ligand-Target Pair
TargetBromodomain testis-specific protein [44-116](Homo sapiens (Human))
Baylor College of Medicine
US Patent
Baylor College of Medicine
US Patent
Affinity DataIC50: 17nMAssay Description:The compounds were screened in vitro, and each compound was serially diluted to 10 concentrations. Four proteins BRD4 (D1+D2), BRDT (D1), BRD2 (D1+D2...More data for this Ligand-Target Pair
Affinity DataIC50: 18nMAssay Description:The compounds were screened in vitro, and each compound was serially diluted to 10 concentrations. Four proteins BRD4 (D1+D2), BRDT (D1), BRD2 (D1+D2...More data for this Ligand-Target Pair
TargetBromodomain testis-specific protein [44-116](Homo sapiens (Human))
Baylor College of Medicine
US Patent
Baylor College of Medicine
US Patent
Affinity DataIC50: 19nMAssay Description:The compounds were screened in vitro, and each compound was serially diluted to 10 concentrations. Four proteins BRD4 (D1+D2), BRDT (D1), BRD2 (D1+D2...More data for this Ligand-Target Pair
Affinity DataIC50: 19nMAssay Description:AlphaScreen is a bead-based, non-radioactive Amplified Luminescent Proximity Homogeneous Assay. The details are not described in this patent.More data for this Ligand-Target Pair
