Reaction Details  Report a problem with these data
Cell Reactant:
Glyoxylate Regulatory Protein (C-AllR)
Syringe Reactant:
BDBM19472
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
12/10/07
ΔG°:
-1.06±n/a (kcal/mole)
pH:
7.4±n/a
Log10Kb:
4.5
Temperature:
298.15±n/a (K)
ΔHobs :
-8.2±n/a (kJ/mole)
Corrected for ΔHioniz:
no
Stoich. Param.:
0.5
ΔS° :
-0.02±n/a (kJ/mole-K)
Citation
 Lorca, GL; Ezersky, A; Lunin, VV; Walker, JR; Altamentova, S; Evdokimova, E; Vedadi, M; Bochkarev, A; Savchenko, A Glyoxylate and pyruvate are antagonistic effectors of the Escherichia coli IclR transcriptional regulator. J Biol Chem 282:16476-91 (2007) [PubMed]  Article
Cell React
Source:
His-tagged fusion protein was overexpressed and purified in E. coli.
Prep. Method:
The His6 tag was cleaved from the protein by tobacco etch virus protease. The cleaved protein was then resolved by flowing the mixture through a second Ni2+ column. The purified proteins were dialyzed and concentrated.
Name:
Glyoxylate Regulatory Protein (C-AllR)
Synonyms:
Negative regulator of allantoin and glyoxylate utilization operons | Transcriptional regulator AllR, C-terminal domain
Type:
C-terminal effector-binding domain
Mol. Mass.:
29267.82
Organism:
Escherichia coli
Description:
P0ACN4
Residue:
271
Sequence:
MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVSDISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVAGPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKALLYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCIASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP
  
Syringe React
Name:
BDBM19472
Synonyms:
2-oxoacetic acid | Glyoxylate | Oxoethanoic acid | glyoxylic acid | oxaldehydic acid
Type:
Small organic molecule
Emp. Form.:
C2H2O3
Mol. Mass.:
74.0355
SMILES:
OC(=O)C=O
Structure:
Search PDB for entries with ligand similarity: