Reaction Details  Report a problem with these data
Cell Reactant:
Acetate Operon Repressor (IclR)
Syringe Reactant:
BDBM19472
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
12/10/07
ΔG°:
-20.9±n/a (kJ/mole)
pH:
7.4±n/a
Log10Kb:
3
Temperature:
298.15±n/a (K)
ΔHobs :
-24.244±n/a (kJ/mole)
Corrected for ΔHioniz:
no
Stoich. Param.:
0.5
ΔS° :
0.0112±n/a (kJ/mole-K)
Citation
 Lorca, GL; Ezersky, A; Lunin, VV; Walker, JR; Altamentova, S; Evdokimova, E; Vedadi, M; Bochkarev, A; Savchenko, A Glyoxylate and pyruvate are antagonistic effectors of the Escherichia coli IclR transcriptional regulator. J Biol Chem 282:16476-91 (2007) [PubMed]  Article
Cell React
Source:
His-tagged fusion protein was overexpressed and purified in E. coli.
Prep. Method:
The His6 tag was cleaved from the protein by tobacco etch virus protease. The cleaved protein was then resolved by flowing the mixture through a second Ni2+ column. The purified proteins were dialyzed and concentrated.
Name:
Acetate Operon Repressor (IclR)
Synonyms:
ICLR_ECOLI | iclR
Type:
Transcriptional regulator
Mol. Mass.:
29746.52
Organism:
Escherichia coli
Description:
n/a
Residue:
274
Sequence:
MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSVALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLLAIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASGAGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHALGLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR
  
Syringe React
Name:
BDBM19472
Synonyms:
2-oxoacetic acid | Glyoxylate | Oxoethanoic acid | glyoxylic acid | oxaldehydic acid
Type:
Small organic molecule
Emp. Form.:
C2H2O3
Mol. Mass.:
74.0355
SMILES:
OC(=O)C=O
Structure:
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