Reaction Details  Report a problem with these data
Cell Reactant:
Cytochrome P450 2B4 Mutant (F206A)
Syringe Reactant:
BDBM19475
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
12/10/07
pH:
7.4±n/a
Temperature:
298.15±n/a (K)
ΔHobs :
-10.9±n/a (kJ/mole)
Corrected for ΔHioniz:
no
Citation
 Wang, BZ; Wang, J; Wu, Q [BRCA1 protein expression in sporadic breast and its clinical significance]. Zhonghua Wai Ke Za Zhi 41:186-8 (2003) [PubMed]
Cell React
Source:
Purified from E. coli.
Prep. Method:
Cold ethanol was added to the protein to a final concentration of 2% just prior to the temperature equilibration, and the sample was loaded into the calorimetric cell.
Name:
Cytochrome P450 2B4 Mutant (F206A)
Synonyms:
2B4dH(H226Y) mutant F206A | CYPIIB4 | N-terminal truncated and modified form of P450 2B4 | P450 types B0 and B1 | P450-LM2
Type:
Enzyme
Mol. Mass.:
54034.11
Organism:
Oryctolagus cuniculus (rabbit)
Description:
F206A was created using 2B4dH(H226Y) as a template. 2B4dH(H226Y) is an N-terminal truncated and modified and C-terminal His-tagged form of P450 2B4 engineered for enhanced solubility and containing a His- 226 to Tyr substitution to prevent dimerization.
Residue:
476
Sequence:
MAKKTSSKGKLPPGPSPLPVLGNLLQMDRKGLLRSFLRLREKYGDVFTVYLGSRPVVVLCGTDAIREALVDQAEAFSGRGKIAVVDPIFQGYGVIFANGERWRALRRFSLATMRDFGMGKRSVEERIQEEARCLVEELRKSKGALLDNTLLFHSITSNIICSIVFGKRFDYKDPVFLRLLDLFFQSASLISSFSSQVFELFSGFLKYFPGTHRQIYRNLQEINTFIGQSVEKHRATLDPSNPRDFIDVYLLRMEKDKSDPSSEFHHQNLILTVLSLFFAGTETTSTTLRYGFLLMLKYPHVTERVQKEIEQVIGSHRPPALDDRAKMPYTDAVIHEIQRLGDLIPFGVPHTVTKDTQFRGYVIPKNTEVFPVLSSALHDPRYFETPNTFNPGHFLDANGALKRNEGFMPFSLGKRICLGEGIARTELFLFFTTILQNFSIASPVPPEDIDLTPRESGVGNVPPSYQIRFLARHHHH
  
Syringe React
Source:
Sigma-Aldrich
Name:
BDBM19475
Synonyms:
4-(4-chlorophenyl)imidazole | 4-CPI | 5-(4-chlorophenyl)-1H-imidazole
Type:
Small organic molecule
Emp. Form.:
C9H7ClN2
Mol. Mass.:
178.618
SMILES:
Clc1ccc(cc1)-c1c[nH]cn1
Structure:
Search PDB for entries with ligand similarity: