Cell Reactant:
BirA Bifunctional Protein Mutant (V219A)
Syringe Reactant:
BDBM19477
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
12/09/07
ΔG°:
-11±0.1 (kcal/mole)
pH:
7.5±0.02
Log10Kb:
7.5
Temperature:
293.15±0.1 (K)
ΔHobs :
-13.3±0.9 (kJ/mole)
Corrected for ΔHioniz:
no
Stoich. Param.:
0.82
ΔS° :
-0.01±0 (kJ/mole-K)
Citation
 Naganathan, SBeckett, D Nucleation of an allosteric response via ligand-induced loop folding. J Mol Biol 373:96-111 (2007) [PubMed]  Article
Cell React
Source:
The BirA variants were expressed in the Escherichia coli strain JM109 transformed with the plasmid pBtac2 carrying the E. coli birA gene (wild-type or mutant) under transcriptional control of the tac.
Purity:
>95%
Prep. Method:
The separation and purification of BirA from other cellular proteins was carried out in NiNTA column and Sepharose chromatography steps.
Name:
BirA Bifunctional Protein Mutant (V219A)
Synonyms:
BirA adenylate-binding loop (ABL) variant
Type:
Repressor variant
Mol. Mass.:
35285.53
Organism:
Escherichia coli
Description:
n/a
Residue:
321
Sequence:
MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVANQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK
  
Syringe React
Name:
BDBM19477
Synonyms:
btnOH-AMP | {[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy}({5-[(4S)-2-oxo-hexahydro-1H-thieno[3,4-d]imidazolidin-4-yl]pentyl}oxy)phosphinic acid
Type:
Nucleoside or nucleotide
Emp. Form.:
C20H30N7O8PS
Mol. Mass.:
559.533
SMILES:
Nc1ncnc2n(cnc12)[C@@H]1O[C@H](COP(O)(=O)OCCCCC[C@@H]2SCC3NC(=O)NC23)[C@@H](O)[C@H]1O |r|
Structure:
Search PDB for entries with ligand similarity: