Cell Reactant:
BirA Bifunctional Protein
Syringe Reactant:
BDBM19478
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
12/19/07
ΔG°:
-13.9±0.4 (kcal/mole)
pH:
7.5±0.2
Log10Kb:
10.1
Temperature:
293.15±0.1 (K)
ΔHobs :
-12.3±0.9 (kJ/mole)
Corrected for ΔHioniz:
no
ΔS° :
0.01±0 (kJ/mole-K)
Citation
 Brown, PHBeckett, D Use of binding enthalpy to drive an allosteric transition. Biochemistry 44:3112-21 (2005) [PubMed]  Article
Cell React
Source:
The BirA variants were expressed in the Escherichia coli strain JM109 transformed with the plasmid pBtac2 carrying the E. coli birA gene (wild-type or mutant) under transcriptional control of the tac
Purity:
>95%
Prep. Method:
The separation and purification of BirA from other cellular proteins was carried out in NiNTA column and Sepharose chromatography steps.
Name:
Bifunctional ligase/repressor BirA
Synonyms:
Biotin operon repressor | Biotin protein ligase (BPL) | Biotin--[acetyl-CoA-carboxylase] synthetase | BirA Bifunctional Protein
Type:
Repressor protein
Mol. Mass.:
35313.58
Organism:
Escherichia coli
Description:
n/a
Residue:
321
Sequence:
MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK
  
Syringe React
Source:
Synthesized and purified in the laboratory.
Name:
BDBM19478
Synonyms:
BTX | bio-5-AMP | biotinyl-5-AMP | {[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy}({5-[(4S)-2-oxo-hexahydro-1H-thieno[3,4-d]imidazolidin-4-yl]pentanoyl}oxy)phosphinic acid
Type:
Nucleoside or nucleotide
Emp. Form.:
C20H28N7O9PS
Mol. Mass.:
573.517
SMILES:
Nc1ncnc2n(cnc12)[C@@H]1O[C@H](COP(O)(=O)OC(=O)CCCC[C@@H]2SCC3NC(=O)NC23)[C@@H](O)[C@H]1O |r|
Structure:
Search PDB for entries with ligand similarity: