Reaction Details  Report a problem with these data
Cell Reactant:
Streptavadin(W79F)
Syringe Reactant:
BDBM12
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
04/30/08
Temperature:
298.45±n/a (K)
ΔHobs :
-26±0.5 (kJ/mole)
Corrected for ΔHioniz:
no
Stoich. Param.:
0.86
Citation
 Chilkoti, A; Boyer, FE; Stayton, PS; Vara Prasad, JV; Domagala, JM; Ellsworth, EL; Gajda, C; Hagen, SE; Markoski, LJ; Tait, BD; Lunney, EA; Palovsky, A; Ferguson, D; Graham, N; Holler, T; Hupe, D; Nouhan, C; Tummino, PJ; Urumov, A; Zeikus, E; Zeikus, G; Gracheck, SJ; Sanders, JM; VanderRoest, S; Brodfuehrer, J; Iyer, K; Sinz, M; Gulnik, SV Molecular Origins of the Slow Streptavidin-Biotin Dissociation Kinetics J Am Chem Soc 117:10622-10628 (1995)    Article
Cell React
Source:
Mutant protein was expressed in a T7 expression system (pET-21a, Novagen Inc.,Madison,WI) as insoluble inclusion bodies.
Prep. Method:
Refolded protein was purified by affinity chromatography with iminobiotin-agarose.
Name:
Streptavadin(W79F)
Synonyms:
n/a
Type:
Biotin-Binding Protein
Mol. Mass.:
18796.50
Organism:
Streptomyces avidinii
Description:
n/a
Residue:
183
Sequence:
MRKIVVAAIAVSLTTVSITASASADPSKDSKAQVSAAEAGITGTWYNQLGSTFIVTAGADGALTGTYESAVGNAESRYVLTGRYDSAPATDGSGTALGWTVAFKNNYRNAHSATTWSGQYVGGAEARINTQWLLTSGTTEANAWKSTLVGHDTFTKVKPSAASIDAAKKAGVNNGNPLDAVQQ
  
Syringe React
Name:
BDBM12
Synonyms:
5-[(3aS,4S,6aR)-2-oxo-hexahydro-1H-thieno[3,4-d]imidazolidin-4-yl]pentanoic acid | Biotin | cid_171548 | cid_253
Type:
Enzyme co-factor
Emp. Form.:
C10H16N2O3S
Mol. Mass.:
244.311
SMILES:
[H][C@]12CS[C@@H](CCCCC(O)=O)[C@@]1([H])NC(=O)N2 |r|
Structure:
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