Cell Reactant:
Serine Hydroxymethyltransferase
Syringe Reactant:
BDBM287
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
03/31/03
ΔG°:
-8.5±n/a (kcal/mole)
Temperature:
298.15±n/a (K)
ΔH° :
9±n/a (kJ/mole)
ΔHobs :
9±n/a (kJ/mole)
Corrected for ΔHioniz:
not known
ΔCp :
-0.62±n/a (kJ/mole)
Citation
 Hom, RKHuang, TGailunas, AFWang, CMamo, SMaras, BFang, LYBarra, DTung, JSWalker, DESchirch, VDavis, DThorsett, EDJewett, NEMoon, JBJohn, V Thermodynamic analysis of the binding of the polyglutamate chain of 5-formyltetrahydropteroylpolyglutamates to serine hydroxymethyltransferase. Biochemistry 37:13536-42 (1998) [PubMed]  Article
Cell React
Source:
purified in the laboratory
Prep. Method:
SHMT, about 6 mg/mL, was dialyzed overnight against 20 mM potassium phosphate buffer at pH 7.0, containing 50 mM glycine and 1 mM DTT. This enzyme was then diluted to the desired concentration with the dialysis buffer.
Name:
Serine Hydroxymethyltransferase
Synonyms:
GLYC_RABIT | SHMT1
Type:
Enzyme
Mol. Mass.:
52984.86
Organism:
Oryctolagus cuniculus (rabbit)
Description:
P07511
Residue:
484
Sequence:
MATAVNGAPRDAALWSSHEQMLAQPLKDSDAEVYDIIKKESNRQRVGLELIASENFASRAVLEALGSCLNNKYSEGYPGQRYYGGTEHIDELETLCQKRALQAYGLDPQCWGVNVQPYSGSPANFAVYTALVEPHGRIMGLDLPDGGHLTHGFMTDKKKISATSIFFESMAYKVNPDTGYIDYDRLEENARLFHPKLIIAGTSCYSRNLDYGRLRKIADENGAYLMADMAHISGLVVAGVVPSPFEHCHVVTTTTHKTLRGCRAGMIFYRRGVRSVDPKTGKEILYNLESLINSAVFPGLQGGPHNHAIAGVAVALKQAMTPEFKEYQRQVVANCRALSAALVELGYKIVTGGSDNHLILVDLRSKGTDGGRAEKVLEACSIACNKNTCPGDKSALRPSGLRLGTPALTSRGLLEKDFQKVAHFIHRGIELTVQIQDDTGPRATLKEFKEKLAGDEKHQRAVRALRQEVESFAALFPLPGLPGF
  
Syringe React
Source:
B. Schircks
Prep. Method:
A concentrated solution of 5-CHO-H4PteGlun was passed through a 1 cm x 10 cm Bio-Gel P-2 column equilibrated with dialysis buffer. The concentration of the solution was determined from its absorbance at 288 nm using an extinction coefficient of 31 500 M -1 cm -1
Name:
BDBM287
Synonyms:
(2S)-2-[(2S)-2-{[4-({[(6S)-2-amino-5-formyl-4-oxo-1,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)phenyl]formamido}-4-formamidobutanoic acid]pentanedioic acid | 5-CHO-H4PteGlu2
Type:
Small organic molecule
Emp. Form.:
C25H30N8O10
Mol. Mass.:
602.5533
SMILES:
Nc1nc2NC[C@H](CNc3ccc(cc3)C(=O)N[C@@H](CCC(=O)N[C@@H](CCC(O)=O)C(O)=O)C(O)=O)N(C=O)c2c(=O)[nH]1
Structure:
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