Cell Reactant:
Heat Shock Protein 90 (Hsp90)
Syringe Reactant:
BDBM20740
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
09/29/09
ΔG°:
-8.3±n/a (kcal/mole)
pH:
8±n/a
Log10Kb:
5
Temperature:
293.15±n/a (K)
ΔHobs :
-7.87±0.1 (kJ/mole)
Corrected for ΔHioniz:
no
ΔS° :
0±n/a (kJ/mole-K)
Citation
 Nilapwar, SWilliams, EFu, CProdromou, CPearl, LHWilliams, MALadbury, JE Structural-thermodynamic relationships of interactions in the N-terminal ATP-binding domain of Hsp90. J Mol Biol 392:923-36 (2009) [PubMed]  Article
Cell React
Source:
His-tagged N-terminal domain of yeast HSP90 was expressed and purified.
Prep. Method:
Protein was purified by using nickel affinity column, and was subsequently concentrated by an (NH4)2SO4 precipitation and purified further using gel filtration chromatography.
Name:
Heat Shock Protein 90 (Hsp90)
Synonyms:
82 kDa heat shock protein | ATP-dependent molecular chaperone HSP82 | HSP82 | HSP82_YEAST | HSP90 | Heat shock protein Hsp90 heat-inducible isoform
Type:
N-terminal domain
Mol. Mass.:
23389.88
Organism:
Saccharomyces cerevisiae (Baker s yeast)
Description:
P02829 aa 1-207
Residue:
207
Sequence:
MASETFEFQAEITQLMSLIINTVYSNKEIFLRELISNASDALDKIRYKSLSDPKQLETEPDLFIRITPKPEQKVLEIRDSGIGMTKAELINNLGTIAKSGTKAFMEALSAGADVSMIGQFGVGFYSLFLVADRVQVISKSNDDEQYIWESNAGGSFTVTLDEVNERIGRGTILRLFLKDDQLEYLEEKRIKEVIKRHSEFVAYPIQL
  
Syringe React
Name:
BDBM20740
Synonyms:
Geldanamycin
Type:
Antibiotic
Emp. Form.:
n/a
Mol. Mass.:
n/a
SMILES:
n/a
Structure:
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