Target
Dimer of Gag-Pol polyprotein [489-587,K509R,V521I,L522F,M525I,L552P,A560V,V571A,L579M]
Ligand
BDBM580
Substrate
HIV Protease Chromogenic Peptide Substrate
Meas. Tech.
Protease Inhibition Assay
pH
4.7±n/a
Temperature
310.15±n/a K
Ki
0.04±0.01 nM
Km
47000±5000 nM
Comments
kcat/Km=0.58 +/-0.07 uM-1sec-1
Citation
 Clemente, JCMoose, REHemrajani, RWhitford, LRGovindasamy, LReutzel, RMcKenna, RAgbandje-McKenna, MGoodenow, MMDunn, BM Comparing the accumulation of active- and nonactive-site mutations in the HIV-1 protease. Biochemistry 43:12141-51 (2004) [PubMed]  Article 
Target
Name:
Dimer of Gag-Pol polyprotein [489-587,K509R,V521I,L522F,M525I,L552P,A560V,V571A,L579M]
Synonyms:
HIV-1 Protease Mutant V6 | HIV-1 protease mutant (K20R V32I L33F M36I L63P A71V V82A L90M) | The post-ritonavir therapy protease variant
Type:
Protein Complex
Mol. Mass.:
n/a
Description:
The HIV-1 PR DNA for all of the HIV LAI variants was cloned, and protein was expressed, purified, and refolded from Escherichia coli strain BL21.
Components:
This complex has 2 components.
Component 1
Name:
Gag-Pol polyprotein [489-587,K509R,V521I,L522F,M525I,L552P,A560V,V571A,L579M]
Synonyms:
HIV-1 Protease Mutant V6 chain A | HIV-1 Protease Mutant V6 chain B | POL_HV1H2 | gag-pol
Type:
Enzyme Subunit
Mol. Mass.:
10841.17
Organism:
Human immunodeficiency virus type 1
Description:
P04585[489-587,K509R,V521I,L522F,M525I,L552P,A560V,V571A,L579M]
Residue:
99
Sequence:
PQVTLWQRPLVTIKIGGQLREALLDTGADDTIFEEISLPGRWKPKMIGGIGGFIKVRQYDQIPIEICGHKVIGTVLVGPTPANIIGRNLMTQIGCTLNF
  
Component 2
Name:
Gag-Pol polyprotein [489-587,K509R,V521I,L522F,M525I,L552P,A560V,V571A,L579M]
Synonyms:
HIV-1 Protease Mutant V6 chain A | HIV-1 Protease Mutant V6 chain B | POL_HV1H2 | gag-pol
Type:
Enzyme Subunit
Mol. Mass.:
10841.17
Organism:
Human immunodeficiency virus type 1
Description:
P04585[489-587,K509R,V521I,L522F,M525I,L552P,A560V,V571A,L579M]
Residue:
99
Sequence:
PQVTLWQRPLVTIKIGGQLREALLDTGADDTIFEEISLPGRWKPKMIGGIGGFIKVRQYDQIPIEICGHKVIGTVLVGPTPANIIGRNLMTQIGCTLNF
  
Inhibitor
Name:
BDBM580
Synonyms:
(4R)-3-[(2S,3S)-2-hydroxy-3-[(3-hydroxy-2-methylphenyl)formamido]-4-phenylbutanoyl]-5,5-dimethyl-N-[(2-methylphenyl)methyl]-1,3-thiazolidine-4-carboxamide | AG1776 | BDBM50288366 | JE-2147 | KNI-764
Type:
Small organic molecule
Emp. Form.:
C32H37N3O5S
Mol. Mass.:
575.718
SMILES:
Cc1ccccc1CNC(=O)[C@H]1N(CSC1(C)C)C(=O)[C@@H](O)[C@H](Cc1ccccc1)NC(=O)c1cccc(O)c1C |r|
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
HIV Protease Chromogenic Peptide Substrate
Synonyms:
n/a
Type:
Peptide
Mol. Mass.:
1420.11
Organism:
n/a
Description:
n/a
Residue:
13
Sequence:
KARVLNPHEANLG