Target
Dimer of Gag-Pol polyprotein [501-599,V583F]
Ligand
BDBM520
Substrate
HIV Protease Chromogenic Peptide Substrate
Meas. Tech.
Protease Inhibition Assay
pH
4.7±n/a
Temperature
310.15±n/a K
Ki
4±1 nM
Km
18000±2000 nM
Comments
kcat/Km=0.40 +/- 0.05 uM-1sec-1
Citation
 Clemente, JCComan, RMThiaville, MMJanka, LKJeung, JANukoolkarn, SGovindasamy, LAgbandje-McKenna, MMcKenna, RLeelamanit, WGoodenow, MMDunn, BM Analysis of HIV-1 CRF_01 A/E protease inhibitor resistance: structural determinants for maintaining sensitivity and developing resistance to atazanavir. Biochemistry 45:5468-77 (2006) [PubMed]  Article 
Target
Name:
Dimer of Gag-Pol polyprotein [501-599,V583F]
Synonyms:
HIV-1 Protease B Subtype Mutant (V82F)
Type:
Protein Complex
Mol. Mass.:
n/a
Description:
HIV-1 protease DNA for all the HIV B and AE variants was cloned, and protein was expressed and purified from Escherichia coli strain BL21.
Components:
This complex has 2 components.
Component 1
Name:
Gag-Pol polyprotein [501-599,V583F]
Synonyms:
HIV-1 Protease B Subtype Mutant (V82F) Chain A | HIV-1 Protease B Subtype Mutant (V82F) Chain B | POL_HV1BR | gag-pol
Type:
Enzyme Subunit
Mol. Mass.:
10843.23
Organism:
Human immunodeficiency virus type 1
Description:
P03367[501-599,V583F]
Residue:
99
Sequence:
PQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMSLPGRWKPKMIGGIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPFNIIGRNLLTQIGCTLNF
  
Component 2
Name:
Gag-Pol polyprotein [501-599,V583F]
Synonyms:
HIV-1 Protease B Subtype Mutant (V82F) Chain A | HIV-1 Protease B Subtype Mutant (V82F) Chain B | POL_HV1BR | gag-pol
Type:
Enzyme Subunit
Mol. Mass.:
10843.23
Organism:
Human immunodeficiency virus type 1
Description:
P03367[501-599,V583F]
Residue:
99
Sequence:
PQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMSLPGRWKPKMIGGIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPFNIIGRNLLTQIGCTLNF
  
Inhibitor
Name:
BDBM520
Synonyms:
1,3-thiazol-5-ylmethyl N-[(2S,3S,5S)-3-hydroxy-5-[(2S)-3-methyl-2-{[methyl({[2-(propan-2-yl)-1,3-thiazol-4-yl]methyl})carbamoyl]amino}butanamido]-1,6-diphenylhexan-2-yl]carbamate | ABT-538 | CHEMBL163 | Norvir | RTV | Ritonavir
Type:
Small organic molecule
Emp. Form.:
C37H48N6O5S2
Mol. Mass.:
720.944
SMILES:
CC(C)[C@H](NC(=O)N(C)Cc1csc(n1)C(C)C)C(=O)N[C@H](C[C@H](O)[C@H](Cc1ccccc1)NC(=O)OCc1cncs1)Cc1ccccc1
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
HIV Protease Chromogenic Peptide Substrate
Synonyms:
n/a
Type:
Peptide
Mol. Mass.:
1420.11
Organism:
n/a
Description:
n/a
Residue:
13
Sequence:
KARVLNPHEANLG