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Target
Dimer of Gag-Pol polyprotein [501-599,V583F]
Ligand
BDBM577
Substrate
HIV Protease Chromogenic Peptide Substrate
Meas. Tech.
Protease Inhibition Assay
pH
4.7±n/a
Temperature
310.15±n/a K
Ki
2.6±0.4 nM
Km
18000±2000 nM
Comments
kcat/Km=0.40 +/- 0.05 uM-1sec-1
Citation
Clemente, JC; Coman, RM; Thiaville, MM; Janka, LK; Jeung, JA; Nukoolkarn, S; Govindasamy, L; Agbandje-McKenna, M; McKenna, R; Leelamanit, W; Goodenow, MM; Dunn, BM Analysis of HIV-1 CRF_01 A/E protease inhibitor resistance: structural determinants for maintaining sensitivity and developing resistance to atazanavir. Biochemistry 45:5468-77 (2006) [PubMed] Article
More Info.:
Target
Name:
Dimer of Gag-Pol polyprotein [501-599,V583F]
Synonyms:
HIV-1 Protease B Subtype Mutant (V82F)
Type:
Protein Complex
Mol. Mass.:
n/a
Description:
HIV-1 protease DNA for all the HIV B and AE variants was cloned, and protein was expressed and purified from Escherichia coli strain BL21.
Components:
This complex has 2 components.
Component 1
Name:
Gag-Pol polyprotein [501-599,V583F]
Synonyms:
HIV-1 Protease B Subtype Mutant (V82F) Chain A | HIV-1 Protease B Subtype Mutant (V82F) Chain B | POL_HV1BR | gag-pol
Type:
Enzyme Subunit
Mol. Mass.:
10843.23
Organism:
Human immunodeficiency virus type 1
Description:
P03367[501-599,V583F]
Residue:
99
Sequence:
PQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMSLPGRWKPKMIGGIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPFNIIGRNLLTQIGCTLNF
Component 2
Name:
Gag-Pol polyprotein [501-599,V583F]
Synonyms:
HIV-1 Protease B Subtype Mutant (V82F) Chain A | HIV-1 Protease B Subtype Mutant (V82F) Chain B | POL_HV1BR | gag-pol
Type:
Enzyme Subunit
Mol. Mass.:
10843.23
Organism:
Human immunodeficiency virus type 1
Description:
P03367[501-599,V583F]
Residue:
99
Sequence:
PQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMSLPGRWKPKMIGGIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPFNIIGRNLLTQIGCTLNF
Inhibitor
Name:
BDBM577
Synonyms:
(3S)-oxolan-3-yl N-[(2S,3R)-4-[(4-aminobenzene)(2-methylpropyl)sulfonamido]-3-hydroxy-1-phenylbutan-2-yl]carbamate | 141W94 | APV | Agenerase | Amprenavir | BDBM50215393 | CHEMBL116 | VX-478
Type:
Small organic molecule
Emp. Form.:
C25H35N3O6S
Mol. Mass.:
505.627
SMILES:
CC(C)CN(C[C@@H](O)[C@H](Cc1ccccc1)NC(=O)O[C@H]1CCOC1)S(=O)(=O)c1ccc(N)cc1 |r|
Substrate
Name:
HIV Protease Chromogenic Peptide Substrate
Synonyms:
n/a
Type:
Peptide
Mol. Mass.:
1420.11
Organism:
n/a
Description:
n/a
Residue:
13
Sequence:
KARVLNPHEANLG