Reaction Details Report a problem with these data
Target
cAMP-dependent protein kinase catalytic subunit alpha [L49I]
Ligand
BDBM2579
Substrate
Kemptide
Meas. Tech.
Kinase Activity Assay
pH
6.8000±n/a
Temperature
298.1500±n/a K
IC50
47±7 nM
Km
10000.0000±n/a nM
Citation
 Bonn, S; Herrero, S; Breitenlechner, CB; Erlbruch, A; Lehmann, W; Engh, RA; Gassel, M; Bossemeyer, D Structural analysis of protein kinase A mutants with Rho-kinase inhibitor specificity. J Biol Chem 281:24818-30 (2006) [PubMed]  Article 
Target
Name:
cAMP-dependent protein kinase catalytic subunit alpha [L49I]
Synonyms:
PKA C-alpha | cAMP-Dependent Protein Kinase (PKA) Mutant PKAR1 (1) | cAMP-dependent protein kinase, alpha-catalytic subunit | KAPCA_BOVIN | PRKACA | cAMP-Dependent Protein Kinase (PKA) Mutant (L49I)
Type:
Enzyme Catalytic Subunit
Mol. Mass.:
40627.77
Organism:
Bovine
Description:
P00517[L49I]
Residue:
351
Sequence:
MGNAAAAKKGSEQESVKEFLAKAKEDFLKKWENPAQNTAHLDQFERIKTIGTGSFGRVMLVKHMETGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVPGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFSEF
  
Inhibitor
Name:
BDBM2579
Synonyms:
CHEMBL388978 | Staurosporine, 8 | Staurosporin, 4 | (2S,3R,4R,6R)-3-methoxy-2-methyl-4-(methylamino)-29-oxa-1,7,17-triazaoctacyclo[12.12.2.1^{2,6}.0^{7,28}.0^{8,13}.0^{15,19}.0^{20,27}.0^{21,26}]nonacosa-8(13),9,11,14(28),15(19),20(27),21(26),22,24-nonaen-16-one | Staurosporine | US9206188, Staurosporine | US9226923, Staurosporine | US20240002365, Compound staurosporine | US11958831, Example Staurosporine | US20240254124, Compound Staurosporine | US20240309004, Compound STAUROSPORINE | US12350272, Compound Staurosporine | US12398142, Compound Staurosporine | US12410173, Compound Staurosporine
Type:
Small organic molecule
Emp. Form.:
C28H26N4O3
Mol. Mass.:
466.20
SMILES:
C[C@@]12[C@@H]([C@@H](C[C@@H](O1)n3c4ccccc4c5c3c6n2c7ccccc7c6c8c5C(=O)NC8)NC)OC
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
Kemptide
Synonyms:
Aurora Peptide Substrate
Type:
Peptide
Mol. Mass.:
773.92
Organism:
n/a
Description:
50 uM ATP as co-substrate.
Residue:
7
Sequence:
LRRASLG