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Target
Cathepsin B
Ligand
BDBM16499
Substrate
BDBM16498
Meas. Tech.
Inhibitory Activity Measurement of Bovine Cathepsin B.
pH
6.0000±n/a
Temperature
313.1500±n/a K
IC50
23±n/a nM
Comments
The second order rate constant, k2 =185000 M-1s-1.
Citation
 Watanabe, D; Yamamoto, A; Tomoo, K; Matsumoto, K; Murata, M; Kitamura, K; Ishida, T Quantitative evaluation of each catalytic subsite of cathepsin B for inhibitory activity based on inhibitory activity-binding mode relationship of epoxysuccinyl inhibitors by X-ray crystal structure analyses of complexes. J Mol Biol 362:979-93 (2006) [PubMed]  Article 
Target
Name:
Cathepsin B
Synonyms:
BCSB | Cathepsin B precursor | Cathepsin B1 | CATB_BOVIN | CTSB
Type:
Enzyme
Mol. Mass.:
36657.87
Organism:
Bovine
Description:
Bovine spleen CB was purchased from Sigma Co. (USA).
Residue:
335
Sequence:
MWRLLATLSCLLVLTSARSSLYFPPLSDELVNFVNKQNTTWKAGHNFYNVDLSYVKKLCGAILGGPKLPQRDAFAADVVLPESFDAREQWPNCPTIKEIRDQGSCGSCWAFGAVEAISDRICIHSNGRVNVEVSAEDMLTCCGGECGDGCNGGFPSGAWNFWTKKGLVSGGLYNSHVGCRPYSIPPCEHHVNGSRPPCTGEGDTPKCSKTCEPGYSPSYKEDKHFGCSSYSVANNEKEIMAEIYKNGPVEGAFSVYSDFLLYKSGVYQHVSGEIMGGHAIRILGWGVENGTPYWLVGNSWNTDWGDNGFFKILRGQDHCGIESEIVAGMPCTHQY
  
Inhibitor
Name:
BDBM16499
Synonyms:
CA inhibitor 2 | EtO-tES-Ile-Ala-OH | CA075 | (2S)-2-[(2S,3S)-2-{[(2S,3S)-3-(ethoxycarbonyl)oxiran-2-yl]formamido}-3-methylpentanamido]propanoic acid | N-{[(2S,3S)-3-(ethoxycarbonyl)oxiran-2-yl]carbonyl}-L-isoleucyl-L-alanine | epoxysuccinyl derivative 2
Type:
Small organic molecule
Emp. Form.:
TBD
Mol. Mass.:
TBD
SMILES:
TBD
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
BDBM16498
Synonyms:
Benzyloxycarbonyl (Z)-Phe-Arg 7-(4-methyl)coumarylamide (-NMec) | CBZ-Phe-Arg-AMC | benzyl N-[(1S)-1-{[(1S)-4-carbamimidamido-1-[(4-methyl-2-oxo-2H-chromen-7-yl)carbamoyl]butyl]carbamoyl}-2-phenylethyl]carbamate | Z-Phe-Arg-NMec
Type:
substrate
Emp. Form.:
TBD
Mol. Mass.:
TBD
SMILES:
TBD
Structure:
Search PDB for entries with ligand similarity: