Reaction Details Report a problem with these data
Target
Complex of Baculoviral IAP repeat-containing protein 7 [1-159,S150G] and E3 ubiquitin-protein ligase XIAP [336-348]
Ligand
BDBM17348
Substrate
BDBM17342
Meas. Tech.
Fluorescence Polarization Affinity Measurements.
pH
7.2000±n/a
Temperature
295.1500±n/a K
Ki
4190±n/a nM
Citation
 Zobel, K; Wang, L; Varfolomeev, E; Franklin, MC; Elliott, LO; Wallweber, HJ; Okawa, DC; Flygare, JA; Vucic, D; Fairbrother, WJ; Deshayes, K Design, synthesis, and biological activity of a potent Smac mimetic that sensitizes cancer cells to apoptosis by antagonizing IAPs. ACS Chem Biol 1:525-33 (2006) [PubMed]  Article 
Target
Name:
Complex of Baculoviral IAP repeat-containing protein 7 [1-159,S150G] and E3 ubiquitin-protein ligase XIAP [336-348]
Synonyms:
ML-IAP-BIR | Chimeric protein of melanoma inhibitor of apoptosis protein and XIAP-BIR3 | MLXBIR3SG
Type:
Chimeric Protein
Mol. Mass.:
19011.07
Organism:
Human
Description:
Amino acids 160-179 of MLBIR were replaced with amino acids 336-348 of XIAP-BIR3, and Ser150 of MLBIR was mutated to glycine to give MLXBIR3SG.
Residue:
172
Sequence:
MGPKDSAKCLHRGPQPSHWAAGDGPTQERCGPRSLGSPVLGLDTCRAWDHVDGQILGQLRPLTEEEEEEGAGATLSRGPAFPGMGSEELRLASFYDWPLTAEVPPELLAAAGFFHTGHQDKVRCFFCYGGLQSWKRGDDPWTEHAKWFPGCQFLLRSKGQEYINNIHLTHSL
  
Inhibitor
Name:
BDBM17348
Synonyms:
(3R,6S,9aS)-N-(3-methyl-1-phenyl-1H-pyrazol-5-yl)-6-[(2S)-2-(methylamino)propanamido]-5-oxo-octahydroazepino[2,1-b][1,3]thiazole-3-carboxamide | peptide isostere, 11
Type:
Small organic molecule
Emp. Form.:
TBD
Mol. Mass.:
TBD
SMILES:
TBD
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
BDBM17342
Synonyms:
5-carboxyflourescein(5-FAM)-conjugated AVPFFAKK | AVP-diPhe-FAM
Type:
Fluorescent dye-conjugated peptide
Emp. Form.:
TBD
Mol. Mass.:
TBD
SMILES:
TBD
Structure:
Search PDB for entries with ligand similarity: