Reaction Details Report a problem with these data
Target
(S)-6-hydroxynicotine oxidase [N166A]
Ligand
BDBM217378
Substrate
n/a
Meas. Tech.
Steady-State Kinetic Assay
pH
8.0000±0.0000
Temperature
571.3000±0.0000 K
Kd
3.10e+5± 5.0e+4 nM
Citation
 Fitzpatrick, PF; Chadegani, F; Zhang, S; Dougherty, V Mechanism of Flavoprotein l-6-Hydroxynicotine Oxidase: pH and Solvent Isotope Effects and Identification of Key Active Site Residues. Biochemistry 56:869-875 (2017) [PubMed]  Article 
Target
Name:
(S)-6-hydroxynicotine oxidase [N166A]
Synonyms:
HLNO_PAENI | 6-hlno | L-6-hydroxynicotine oxidase (LHNO N166A)
Type:
Enzyme
Mol. Mass.:
46274.19
Organism:
Arthrobacter nicotinovorans
Description:
A. nicotinovorans LHNO N166A mutant
Residue:
425
Sequence:
MYDAIVVGGGFSGLKAARDLTNAGKKVLLLEGGERLGGRAYSRESRNVPGLRVEIGGAYLHRKHHPRLAAELDRYGIPTAAASEFTSFRHRLGPTAVDQAFPIPGSEAVAVEAATYTLLRDAHRIDLEKGLENQDLEDLDIPLNEYVDKLDLPPVSRQFLLAWAWAMLGQPADQASALWMLQLVAAHHYSILGVVLSLDEVFSNGSADLVDAMSQEIPEIRLQTVVTGIDQSGDVVNVTVKDGHAFQAHSVIVATPMNTWRRIVFTPALPERRRSVIEEGHGGQGLKILIHVRGAEAGIECVGDGIFPTLYDYCEVSESERLLVAFTDSGSFDPTDIGAVKDAVLYYLPEVEVLGIDYHDWIADPLFEGPWVAPRVGQFSRVHKELGEPAGRIHFVGSDVSLEFPGYIEGALETAECAVNAILHS
  
Inhibitor
Name:
BDBM217378
Synonyms:
(S)-6-hydroxynicotine | 5-[(2S)-1-methylpyrrolidin-2-yl]-1H-pyridin-2-one
Type:
Small organic molecule
Emp. Form.:
n/a
Mol. Mass.:
n/a
SMILES:
CN1CCC[C@H]1c1ccc(=O)[nH]c1
Structure:
Search PDB for entries with ligand similarity: