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Target
DNA polymerase alpha subunit B
Ligand
BDBM33387
Substrate
DNA template-primer
Meas. Tech.
DNA Polymerase Assay
pH
7.5±n/a
Temperature
310.15±n/a K
IC50
90±n/a nM
Citation
 Kuramochi, K; Fukudome, K; Kuriyama, I; Takeuchi, T; Sato, Y; Kamisuki, S; Tsubaki, K; Sugawara, F; Yoshida, H; Mizushina, Y Synthesis and structure-activity relationships of dehydroaltenusin derivatives as selective DNA polymerase alpha inhibitors. Bioorg Med Chem 17:7227-38 (2009) [PubMed]  Article 
Target
Name:
DNA polymerase alpha subunit B
Synonyms:
70 kD subunit | DNA Polymerase alpha | DNA polymerase alpha 70 kDa subunit | DNA polymerase alpha subunit B | DPOA2_BOVIN | POLA2 | Polymerase (DNA directed), alpha 2
Type:
Enzyme Subunit
Mol. Mass.:
66940.74
Organism:
Bos taurus (bovine)
Description:
Enzyme was purchased from Ulrich Hubscher, University of Zurich-Irchel, Switzerland.
Residue:
604
Sequence:
MAVSAQLLVEELQIFGLECEEAVIEKLVELCILYGQNEEGMASELIAFCTSTRKDCFTLETLNSFEHEFLSKRVSKTRHGASKDKGLRHAGARDIVSIQELIEVEEEEETLLNSYTTPSKGSQKRTITTPETPLTKRSVSARSPHQLLSPSSFSPSATPPQKYSSRSNRGEVVTSFGSAQGVSWSGRGGASPLSLKVLGHPEPLTGSYKYMFQKLPDIREVLTCKIEELGSELKEHYKIEAFAPILVPAQEPVTLLGQIGCDSNGKLNHKSVILEGDLEHSSGAQIPVDLSELKEYSLFPGQVVVMEGINTTGRKLVATRLYEGVPLPFHQPDEEDGDSEQFMVLVACGPYTTSDSITFDPLLDLITIINRDRPDVCILFGPFLDAKHEQVESCLLTSSFEDVFKQCLRTIIEGTRSSGSHLIIVPSLRDVHHEPVYPQPPFSCSDLLREDKKRVRLVSEPCTLSINGVIFGLTSTDLLFHMGAEEISSSSGTSDRFSRILRHILTQRSYYPLYPPQEDMAIDYENFYLYAQLPVTPDVFIAPSELRYFVKVGLNSAISLQCQNQFLSGALALNSSRVCWWGQWPRSFGGGVGVENKSVSIMLK
  
Inhibitor
Name:
BDBM33387
Synonyms:
dehydroaltenusin derivative, 15
Type:
Small organic molecule
Emp. Form.:
C20H22O6
Mol. Mass.:
358.3851
SMILES:
CCCCCCOc1cc(O)c2C(=O)OC3(C)CC(=O)C(=O)C=C3c2c1 |c:22|
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
DNA template-primer
Synonyms:
poly(dA)/oligo(dT)(12Â?1), A/T = 2/1
Type:
Duplex DNA
Mol. Mass.:
358.43
Organism:
n/a
Description:
[3H]-dTTP as co-substrate.
Residue:
3
Sequence:
NA