Reaction Details Report a problem with these data
Target
Protein Mdm4
Ligand
BDBM35076
Substrate
p53 Peptide
Meas. Tech.
Fluorescence Anisotropy Competition Binding Assay
IC50
4100±n/a nM
Citation
 Hayashi, R; Wang, D; Hara, T; Iera, JA; Durell, SR; Appella, DH N-acylpolyamine inhibitors of HDM2 and HDMX binding to p53. Bioorg Med Chem 17:7884-93 (2009) [PubMed]  Article 
Target
Name:
Protein Mdm4
Synonyms:
Double minute 4 protein | Double minute 4 protein (HDM4) | MDM2-MDMX | MDM4 | MDM4_HUMAN | MDMX | Mdm2-like p53-binding protein | Protein Mdm4 | Protein Mdmx | p53-Binding Protein HDMX | p53-binding protein | p53-binding protein Mdm4
Type:
Oncoprotein
Mol. Mass.:
54842.04
Organism:
Homo sapiens (Human)
Description:
The HDMX(1-188) were expressed in Escherichia coli BL21 (DE3) as a GST fusion and purified on glutathione sepharose.
Residue:
490
Sequence:
MTSFSTSAQCSTSDSACRISPGQINQVRPKLPLLKILHAAGAQGEMFTVKEVMHYLGQYIMVKQLYDQQEQHMVYCGGDLLGELLGRQSFSVKDPSPLYDMLRKNLVTLATATTDAAQTLALAQDHSMDIPSQDQLKQSAEESSTSRKRTTEDDIPTLPTSEHKCIHSREDEDLIENLAQDETSRLDLGFEEWDVAGLPWWFLGNLRSNYTPRSNGSTDLQTNQDVGTAIVSDTTDDLWFLNESVSEQLGVGIKVEAADTEQTSEEVGKVSDKKVIEVGKNDDLEDSKSLSDDTDVEVTSEDEWQCTECKKFNSPSKRYCFRCWALRKDWYSDCSKLTHSLSTSDITAIPEKENEGNDVPDCRRTISAPVVRPKDAYIKKENSKLFDPCNSVEFLDLAHSSESQETISSMGEQLDNLSEQRTDTENMEDCQNLLKPCSLCEKRPRDGNIIHGRTGHLVTCFHCARRLKKAGASCPICKKEIQLVIKVFIA
  
Inhibitor
Name:
BDBM35076
Synonyms:
N-acylpolyamine (NAPA) scaffold, 23
Type:
Small organic molecule
Emp. Form.:
C54H85Br2N9O9
Mol. Mass.:
1164.116
SMILES:
CC(C)CCC(=O)NC(CCC(O)=O)CN(C(CCCCN)CN(C(CCC(O)=O)CN(CCC(N)=O)C(=O)NCCCc1ccc(Br)cc1)C(=O)NCCc1ccc(Br)cc1)C(=O)NCCCC1CCCCC1
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
Cellular tumor antigen p53 (aa 15-29)
Synonyms:
P53 | P53_TUPBE | TP53 | p53 (aa 15-29)
Type:
fluorescein-conjugated peptide
Mol. Mass.:
1804.95
Organism:
Tupaia belangeri (Common tree shrew) (Tupaia glis belangeri)
Description:
Q9TTA1 aa 15-29
Residue:
15
Sequence:
SQETFSDLWKLLPEN